Centrifugation is a method of separating, concentrating, and purifying biological samples using the centrifugal force of rotational motion and the difference in sedimentation coefficient or buoyancy density of the material. Its principle of action is centrifugal filtration and centrifugal sedimentation.
1. Centrifugal filtration is the centrifugal pressure generated by the suspension under the centrifugal field. It acts on the filter medium, so that the liquid passes through the filter medium as the filtrate, and the solid particles are trapped on the surface of the filter medium, thereby achieving solid-liquid separation.
2. Centrifugal sedimentation is the principle of rapid sedimentation and stratification in the centrifugal force field by using different components of suspension (or emulsion) density to achieve liquid-solid (or liquid-liquid) separation.
The centrifuge is a powerful centrifugal force generated by the high-speed rotation of the centrifuge rotor, forcing the particles in the liquid to overcome the diffusion and accelerate the sedimentation speed, and separate the substances with different sedimentation coefficients and buoyancy densities in the sample.